A method for simultaneous detection of multiple mRNAs using digoxigenin and radioisotopic cRNA probes.
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چکیده
منابع مشابه
In situ hybridization with non-radioactive digoxigenin-11-UTP-labeled cRNA probes: localization of developmentally regulated mouse tenascin mRNAs.
An improved method for in situ hybridization was developed in order to identify the tissue-specific expression of messenger RNA (mRNA) for the novel extracellular matrix glycoprotein, tenascin, during mouse development. Non-radioactive RNA probes were generated by incorporating digoxigenin-11-UTP instead of conventional isotopic labels. Hybridization of anti-sense probes to complementary mRNAs ...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1993
ISSN: 0022-1554,1551-5044
DOI: 10.1177/41.12.8245421